You work in a laboratory that studies the molecular biology of tribbles. [Although tribbles are an alien life form, assume here that the molecular biology of tribbles is identical to that of eukaryotes on Earth.]Your lab has a genomic library of tribble DNA, as well as a cDNA library made with mRNA extracted from whole tribbles. The lab also has a collection of live tribbles that can be used to isolate RNA or DNA, and a supply of fixed tribbles that can be stained for gene expression.Your advisor provides you with a cloned 100 bp DNA fragment that represents part of the protein-coding region of a tribble gene. Using the tools described in the previous paragraph and the molecular biology techniques we have discussed in class, how would you accomplish each of the following aims? Note: try to come up with the simplest and modest direct approach that will give you the desired information.A. Determine the amino acid sequence of the complete protein produced by that gene.B. Determine whether or not the gene contains introns.C. Determine whether the RNA produced by that gene experiences alternative splicing.D. Determine the length of the mature mRNA(s) produced by the gene. This includes the UTRs and the poly-A tail.E. Determine which cells in the tribble body do and do not express mRNA from this gene.F. You discover a blood stain in the lab, and you want to determine whether it is human blood or tribble blood. How can you do this using the molecular biology tools described above?
A) to determine amino acid sequence of the protein produced by that gene. We will use cDNA library, we will hybridize given part of DNA sequence ( as this part only contains exon part). Than we will isolate the hybridize part and translate this sequence using generic coding table.
B) for determine presence or absence of introns in gene used isolated cDNA in first question. Now we will add this cDNA to DNA library. Here cDNA due to complementary mature binds with DNA. If cDNA binds completely with gene with out looping part of gene it shows that gene is having only exons .
And if along with hybridization part some looped part present in between-- it shows both exons and intron are present.
C) for determining alternative splicing we will use cDNA library.
d) to determine length of mature mRNA which includes both the UTR and poly A sequence we will go for cDNA cloning and look for particular cDNA complementary to DNA segments. And later we isolate that cDNA and examine its whole length
E) to determine which cells in the tribble body express this particular mRNA . We use fluorescent tagged small DNA part provided. Then we will add this DNA probe to supplied tribes. The cells which are expressing , will have cDNA will bind to probe and florescent can be detected. Cells which are not expressing that gene, here probe will not bind and no fluorescence.
F) to determine that whose blood strain is this. We will do VBTR profiling . Which VNTR profiling similar to belief stain help to determine which blood stain is this.
Genetic and biochemical analyses have shown that chimps are more closely related to humans than they are to orangutans.a. True b. False
Yes it is true that chimps are closely related to human.
Chimps ,bonobos and gorillas have same genetic and biochemical function like humans.That is why they are also called human ancestor.Some of chimps could able to transfer their body according to the environment and slowly slowly become human,but some of them could not able to transfer their body and they are still chimps.
Please select the word from the list that best fits the definitionwhere the energy in food is stored until it is released
The correct answer is - Mitochondria.
Mitochondria is the location that stores the energy in food until it is released. It is the cellular organelle that is the place where the cellular respiration process takes place.
In this organelle, the stored food is converted into a usable energy form, ATP. Mitochondria is also known as the powerhouse of the cell as it converts and releases the energy from the stored sugar or food.
Thus, the correct answer is - mitochondria.
DNA and RNA are both composed of nucleotides. However, RNA has three key differences. Please select thethree items that are unique to RNA.
1.Double stranded 2.Single stranded 3.Contains the nitrogen base Uracil in place of Thymine 4.Backbone contains the sugar ribose 5.Backbone contains the sugar deoxyribose 6.Contains the nitrogen base Thymine in place of Uracil
Three items that are unique to RNA are as follows:
3. Contains the nitrogen base Uracil in place of Thymine.
4. Backbone contains the sugar ribose.
What is bacteria #2
Bacteria are unicellular microorganisms that are not visible by the naked eye. they are found everywhere and exist in millions in population. some of them are harmful while some are essential for us like lactobacillus which helps in formation of curd.
Cells that secrete hormones into the bloodstream which are then carried away to target cells are referred to as __________ cells.a. paracrine b. endocrine c. autocrine d. exocrine
Paracrine cells are the ones that secrete a sign directly to its neighbors, to the extra cell space and not to the blood.
Endocrine cells are the ones that secrete a hormone to the blood system to be distributed to some specific place in the body.
The autocrine system is the release of a hormone from the cell to that same cell.
The exocrine glands are those who release a specific type of hormone or enzyme to some part of the body, through canals.
The gene for hemophilia in humans is present on the X chromosome only. Which inheritance pattern does hemophilia in humans represent?Homozygous Multiple alleles Polygenic Sex linked
i think its sex linked but im not sure.
If it is an X-Linked trait, the answer would have to be "Sex-Linked". If it is on the X chromosome, it is on a sex chromosome.